|
KMID : 0545120150250101728
|
|
Journal of Microbiology and Biotechnology
2015 Volume.25 No. 10 p.1728 ~ p.1733
|
|
|
Real-Time Monitoring of Catheter-Related Biofilm Infection in Mice
|
|
Liu Xu
Yin Hong
Xu Xian Xing
Cheng Yuan Guo
Cai Yun
Wang Rui
|
|
|
Abstract
|
|
|
|
This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BLI. The numbers of integrated brightness (IB) and viable bacterial count (VBC) in the biofilm disks in vitro were highest at 24 h after inoculation; the IB of biofilm in vivo was increased until 24 h after implantation. A statistical correlation was observed between IB and VBC in vitro by linear regression analysis. The actual VBC value in vivo can be estimated accurately by IB without sacrifice. In addition, we monitored the change in white blood cells (WBCs) during infection. The number of WBCs on day 7 was significantly higher in the infection group than in the control group. This study indicates that BLI is a simple, fast, and sensitive method to measure catheter biofilm infection in mice.
|
|
|
KEYWORD
|
|
|
bioluminescence imaging, biofilm, catheter-related infection, in vivo
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|